Basics of Flow cytometry: from principles to analysis course

Course Description

We are organizing a post-graduate flow cytometry course at the University of Eastern Finland, Kuopio on 7.-9.12.2022. To our delight, flow cytometry expert Derek Davies (The Francis Crick Institute, London, UK) has agreed to give talks at the course. The schedule consists of two seminar days 7.-8.12.2022, in which the main topic will be basics of flow cytometry and different flow cytometry applications.  The seminar days are aimed at PhD students, post-doctoral researchers and senior researchers interested in learning about flow cytometry. The hands-on demos will be offered to selected PhD students on 9th of December.

Registration

This course is free of charge for university students and UEF faculty members, but registration is required as the number of participants is limited to 50 individuals for the seminar days. Registration fee for other participants (from companies) is 100 €. We will contact participants from companies separately for payment information. For the demo day, we will select a limited amount of interested PhD students based on the motivation letter that needs to be submitted during registration. Details related to the motivation letter are found in registration form. The course is aimed for PhD students, post-doctoral researchers and senior researchers/faculty members who are interested in the topic. 

Credit points for PhD students:

• 1 credit point for attendance in both seminar days
• 1.5 credit points for attendance in both seminar days and demo

Schedule

Day one – 7th December 

08:45-09:15    Intro and overview 
09:15-09:45    Fluorescence and Fluorochromes 
09:45-10:30    The Cytometer – how it works
 
10:30-11:00    Break 
 
11:00-11:45    Compensation 
11:45-12:30    Sponsor 1 Nordic Biosite
 
12:30-13:15    Lunch 
 
13:15-14:15    Experimental Planning  
14:15-15:00    Sample prep and running 
 
15:00-15:30    Break 
 
15:30-16:15    Alternative technologies (Mass, imaging and spectral cytometry) 

Day two – 8th December 

09:00-09:30    Controls and optimisation 
09:30-10:30    Applications 1 – Proliferation and cell death
 
10:30-11:00    Break 
 
11:00-11:45    Sponsor 2 BD Biosciences
11:45-12:30    Applications 2 – Fluorescent proteins, functional assays
 
12:30-13:15    Lunch 
 
13:15-14:45    Data Analysis – principles and demo including compensation
 
14:45-15:15    Break 
 
15:15-15:45    Data presentation 
15:45-16:00    Wrap-up     
 
Applications will include DNA, proliferation, functional assays, fluorescent proteins.

Day three – 9th December

DEMO day for selected PhD students according to the motivation letter. 

8:00-16:00 Demos/hands-on sessions in small groups about:

• How to operate flow cytometers
• Perform flow cytometric stainings and/or assays
• Demo on flow sorting

Specific details on demos are given closer to the course date.

Small changes to the program are possible.